S/MARt DB - S/MARbinder

AC  SB000034
XX
DT  2.2.1999 10:00:06 (created); ili
DT  5.3.2001 18:09:00 (updated); ili
XX
NA  MATH20
XX
SY  multi-AT-hook protein 20 
XX
OS  empty
OC  empty
XX
SZ  1358 AA; 144.4 kDa (cDNA), 83.6 kDa (SDS) [2]
IP  11.6 (cal)
XX
SQ  SSESSSKSSQPLASKQEKDGTEKRGRGRPRKQPPVSPGTALVGSQKEPSEVPTPKRPRGR
SQ  PKGSKNGRSSESSSKSSQPLASKQEKDGTEKRGRGRPRKQPPVSPGTALVGSQKEPSEVP
SQ  TPKRPRGRPKGSKNGRSSESSSKSSQPLASKQEKDGTEKRGRGRPRKQPPVSPGTALVGS
SQ  QKEPSEVPTPKRPRGRPKGSKNGRSSESSSKSSQPLASKQEKDGTEKRGRGRPRKQPPVS
SQ  PGTALVGSQKEPSEVPTPKRPRGRPKGSKNGRSSESSSKSSQPLASKQEKDGTEKRGRGR
SQ  PRKQPPVSPGTALVGSQKEPSEVPTPKRPRGRPKGSKNGRSSESSSKSSQPLASKQEKDG
SQ  TEKRGRGRPRKQPPVSPGTALVGSQKEPSEVPTPKRPRGRPKGSKNGRSSESSSKSSQPL
SQ  ASKQEKDGTEKRGRGRPRKQPPVSPGTALVGSQKEPSEVPTPKRPRGRPKGSKNGRSSES
SQ  SSKSSQPLASKQEKDGTEKRGRGRPRKQPPVSPGTALVGSQKEPSEVPTPKRPRGRPKGS
SQ  KNGRSSESSSKSSQPLASKQEKDGTEKRGRGRPRKQPPVSPGTALVGSQKEPSEVPTPKR
SQ  PRGRPKGSKNGRSSESSSKSSQPLASKQEKDGTEKRGRGRPRKQPPVSPGTALVGSQKEP
SQ  SEVPTPKRPRGRPKGSKNGRSSESSSKSSQPLASKQEKDGTEKRGRGRPRKQPPVSPGTA
SQ  LVGSQKEPSEVPTPKRPRGRPKGSKNGRSSESSSKSSQPLASKQEKDGTEKRGRGRPRKQ
SQ  PPVSPGTALVGSQKEPSEVPTPKRPRGRPKGSKNGRSSESSSKSSQPLASKQEKDGTEKR
SQ  GRGRPRKQPPVSPGTALVGSQKEPSEVPTPKRPRGRPKGSKNGRSSESSSKSSQPLASKQ
SQ  EKDGTEKRGRGRPRKQPPVSPGTALVGSQKEPSEVPTPKRPRGRPKGSKNGRSSESSSKS
SQ  SQPLASKQEKDGTEKRGRGRPRKQPPVSPGTALVGSQKEPSEVPTPKRPRGRPKGSKNGR
SQ  SSESSSKSSQPLASKQEKDGTEKRGRGRPRKQPPVSPGTALVGSQKEPSEVPTPKRPRGR
SQ  PKGSKNGRSSESSSKSSQPLASKQEKDGTEKRGRGRPRKQPPVSPGTALVGSQKEPSEVP
SQ  TPKRPRGRPKGSKNGRSSESSSKSSQPLASKQEKDGTEKRGRGRPRKQPPVSPGTALVGS
SQ  QKEPSEVPTPKRPRGRPKGSKNGRSSESSSKSSQPLASKQEKDGTEKRGRGRPRKQPPVS
SQ  PGTALVGSQKEPSEVPTPKRPRGRPKGSKNGRSSESSSKSSQPLASKQEKDGTEKRGRGR
SQ  PRKQPPVSPGTALVGSQKEPSEVPTPKRPRGRPKGSKN
SC  [2]
XX
FT  2 - 66: repetitve motif containing 2 AT-hooks and a putative
FT          cdc2 phosphorylation site 
XX
SF  given sequence is incomplete; synthetic multi-AT-hook
SF  protein, consisting of 20 reiterated AT-hook peptide motifs
SF  derived from HMG I [3] [2]
XX
FF  highly specific SAR binding (13303]; inhibits chromosome
FF  condensation [2]; acts through A tracts [2];
FF  expression in Drosophila led to a suppression of position
FF  effect variegation [3]; interferes with topoisomerase II
FF  cleavage in satellite III of Drosophila [3]
XX
BS  SM0000037; DROME$H1H3 [2]
MM  direct gel shift;
PR  310 bp subfragment
SO  rec; 
QA  6
BS  SM0000118; DROME$SatIII [3]
MM  topoisomerase II cleavage assay;
SO  rec; 
QA  6
BS  SM0000124; DROME$sgs4-5MAR [1]
MM  sedimentation assay;
SO  rec(S.pombe-S.pombe); Schizosaccharomyces pombe
QA  6
XX
RN  [1]
RX  MEDLINE; 90076119 PubMed; 2556260
RA  Adachi, Y., Kaes, E., Laemmli, U. K.
RT  Preferential, cooperative binding of DNA topoisomerase II to
RT  scaffold-associated regions
RL  EMBO J. 8:3997-4006 (1989)
RN  [2]
RX  MEDLINE; 96128118 PubMed; 8548801
RA  Strick, R., Laemmli, U. K.
RT  SARs are cis DNA elements of chromosome dynamics: synthesis
RT  of a SAR repressor protein
RL  Cell 83:1137-1148 (1995)
RN  [3]
RX  MEDLINE; 98190088 PubMed; 9524129
RA  Girard, F., Bello, B., Laemmli, U. K., Gehring, W. J.
RT  In vivo analysis of scaffold-associated regions in
RT  Drosophila: a synthetic high-affinity SAR binding protein
RT  suppresses position effect variegation
RL  EMBO J. 17:2079-2085 (1998)
//