S/MARt DB - S/MARbinder

AC  SB000041
XX
DT  8.3.1999 15:24:14 (created); ili
DT  19.2.2002 19:10:00 (updated); ili
XX
NA  p130
XX
OS  rat, Rattus spec.
OC  eukaryota; animalia; metazoa; chordata; vertebrata;
OC  tetrapoda; mammalia; eutheria; rodentia; myomorpha; muridae;
OC  murinae
XX
SZ  130 kDa (SDS) [3]; 120 kDa (sed.) [2]; 120 kDa
SZ  (dephosphorylated, SDS) [5]
IP  8.1 (exp.) [2]
XX
SF  immunologically related to p230 SB000040 and to p123
SF  SB000042 [3]; hyperphosphorylated [4]; different
SF  phosphorylation status is supposed account for the
SF  difference in the apparent molecular weight compared to p123
SF  SB000042 [5]; phosphorylated on serine, threonine and
SF  tyrosine residues [5]
XX
FF  recognize bend DNA [4]; no self-aggregation [2];
FF  complex with RAT$MAR1 SM0000134 is believed  to consist of
FF  1 protein molecule and 2 S/MAR fragments [2]; binding
FF  activity is about three times higher than that of p123
FF  SB000042 [5]; phosphoryl groups are supposed to be
FF  involved in alteration of the DNA binding activities [5];
FF  binding affinity is dependent on the methylation status of
FF  the DNA [4]; binds un- or less methylated DNA [4]
XX
CP  rat liver, Ac2F [3]
XX
BS  SM0000134; RAT$MAR1 [1]
MM  DNase I footprinting;
SO  rl; rat
QA  6
BS  SM0000134; RAT$MAR1 [3]
MM  southwestern blotting;
SO  rl; rat
QA  6
BS  SM0000134; RAT$MAR1 [2]
MM  replacement by distamycin A;
SO  rl; rat
QA  6
BS  SM0000135; RAT$MAR2 [3]
MM  nitrocellulose filter binding;
SO  rl; rat
QA  6
BS  SM0000135; RAT$MAR2 [3]
MM  replacement by distamycin A;
SO  rl; rat
QA  6
XX
RN  [1]
RX  MEDLINE; 94071964 PubMed; 8250943
RA  Hibino, Y., Tsukada, S., Sugano, N.
RT  Properties of a DNA-binding protein from rat nuclear
RT  scaffold fraction
RL  Biochem. Biophys. Res. Commun. 197:336-342 (1993)
RN  [2]
RX  MEDLINE; 93363634 PubMed; 8357833
RA  Hibino, Y., Nakamura, K., Sugano, N.
RT  Purification and characterization of nuclear scaffold
RT  proteins which bind to a highly repetitive bent DNA from rat
RT  liver.
RL  Biochim. Biophys. Acta 1174:162-170 (1993)
RN  [3]
RX  MEDLINE; 97265267 PubMed; 9111204
RA  Hibino, Y., Tsukada, S., Sugano, N.
RT  Purification and characterization of a DNA binding protein
RT  in a nuclear scaffold fraction from rat ascites hepatoma
RT  cells
RL  Carcinogenesis 18:707-713 (1997)
RN  [4]
RX  MEDLINE; 99045399 PubMed; 9826523
RA  Hibino, Y., Ohzeki, H., Hirose, N., Morita, Y., Sugano, N.
RT  Involvement of DNA methylation in binding of a highly
RT  repetitive DNA component to nuclear scaffold proteins from
RT  rat liver
RL  Biochem. Biophys. Res. Commun. 252:296-301 (1998)
RN  [5]
RX  MEDLINE; 98193131 PubMed; 9524232
RA  Hibino, Y., Ohzeki, H., Hirose, N., Sugano, N.
RT  Involvement of phosphorylation in binding of nuclear
RT  scaffold proteins from rat liver to a highly repetitive DNA
RT  component
RL  Biochim. Biophys. Acta 1396:88-96 (1998)
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