S/MARt DB - S/MARbinder

AC  SB000046
XX
DT  30.6.1999 11:54:40 (created); ili
XX
NA  ACBP
XX
SY  ARS consensus-binding protein 
XX
OS  yeast, Saccharomyces cerevisiae
OC  Eukaryota; Fungi; Ascomycota; Hemiascomycetes;
OC  Saccharomycetales; Saccharomycetaceae; Saccharomyces.
XX
SZ  67-75 kDa (SDS) [1]
XX
FF  sequence specific binding of the T-rich single strand of
FF  yeast ARS consensus [1]; recognizes also double stranded
FF  yeast ARS consensus [1]
XX
BS  SM0000115; Y$H4-ARS [1]
MM  direct gel shift;
SO  yeast, extract; baker's yeast, Saccharomyces cerevisiae
QA  6
BS  SM0000115; Y$H4-ARS [1]
MM  gel shift competition;
PR  T-rich strand of the ARS oligonucleotide
SO  yeast, extract; baker's yeast, Saccharomyces cerevisiae
QA  6
BS  SM0000115; Y$H4-ARS [1]
MM  southwestern blotting;
PR  T-rich strand of the ARS oligonucleotide
SO  yeast, extract; baker's yeast, Saccharomyces cerevisiae
QA  6
BS  SM0000115; Y$H4-ARS [1]
MM  UV-crosslinking;
PR  T-rich strand of the ARS oligonucleotide
SO  yeast, extract; baker's yeast, Saccharomyces cerevisiae
QA  6
BS  SM0000120; Y$ARS1 [1]
MM  direct gel shift;
PR  single stranded probes: Hind III/Rsa I, Hind III/Bgl II,
PR  Hind III/Pst I
SO  yeast, extract; baker's yeast, Saccharomyces cerevisiae
QA  6
XX
RN  [1]
RX  MEDLINE; 91160059 PubMed; 2001590
RA  Hofmann, J. F., Gasser, S. M.
RT  Identification and purification of a protein that binds the
RT  yeast ARS consensus sequence
RL  Cell 64:951-960 (1991)
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