S/MARt DB - S/MARbinder

AC  SB000054
XX
DT  6.12.1999 17:03:20 (created); ili
DT  14.8.2000 18:55:12 (updated); ili
XX
NA  Ku autoantigen
XX
OS  human, Homo sapiens
OC  eukaryota; animalia; metazoa; chordata; vertebrata;
OC  tetrapoda; mammalia; eutheria; primates
XX
SF  subunit of DNA-PK SB000055 [2]; by itself a
SF  heterodimer composed of a p70 and a p86 subunit [1];
SF  ribosylated by PARP SB000062 [2]
XX
FF  subunit of DNA-dependent protein kinase and by itself a
FF  heterodimer [1]; kown to bind to free ends of DNA
FF  [1]; may also bind to DNA in sequence-dependent manner
FF  [1]; concluded to recognize the primary sequence of base
FF  unpairing regions [1]; binds also to the IgH enhancer
FF  at elevated concentrations [1]; ADP-ribosylation
FF  circumvents binding to base unpairing regions [1];
FF  physical interaction with PARP in vivo is independent of the
FF  presence of DNA and critical for synergistic binding to
FF  S/MARs [1]; involved in chromosome condensation [2]
XX
IN  SB000062; PARP
XX
CP  SK-BR3 [1]
XX
BS  SM0000044; MOUSE$IgHmu-5MAR [1]
MM  direct gel shift;
SO  SK-BR 3; human
QA  6
BS  SM0000044; MOUSE$IgHmu-5MAR [1]
MM  assessment of the dissociation constant;
MM  0.2 nM
SO  SK-BR 3; human
QA  6
BS  SM0000045; MOUSE$IgHmu-3MAR [1]
MM  direct gel shift;
SO  SK-BR 3; human
QA  6
BS  SM0000045; MOUSE$IgHmu-3MAR [1]
MM  assessment of the dissociation constant;
MM  0.05 nM
SO  SK-BR 3; human
QA  6
BS  SM0000046; AS$MAR [1]
MM  affinity chromatography;
PR  (25)7
SO  SK-BR 3; human
QA  6
BS  SM0000046; AS$MAR [1]
MM  direct gel shift;
PR  (25)7
SO  SK-BR 3; human
QA  6
XX
RN  [1]
RX  MEDLINE; 99329069 PubMed; 10400681
RA  Galande, S., Kohwi-Shigematsu, T.
RT  Poly(ADP-ribose) polymerase and Ku autoantigen form a
RT  complex and synergistically bind to matrix attachment
RT  sequences
RL  J. Biol. Chem. 274:20521-20528 (1999)
RN  [2]
RX  MEDLINE; 20271503 PubMed; 10813395
RA  Galande, S., Kohwi-Shigematsu, T.
RT  Caught in the act: binding of Ku and PARP to MARs reveals
RT  novel aspects of their functional interaction
RL  Crit. Rev. Eukaryot. Gene Expr. 10:63-72 (2000)
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