S/MARt DB - S/MAR

AC  SM0000108
XX
DT  1.1.1999 00:00:00 (created); ili
DT  27.2.2002 15:31:00 (updated); ili
XX
NA  HS$apoB-5MAR2
XX
OS  human, Homo sapiens
OC  eukaryota; animalia; metazoa; chordata; vertebrata;
OC  tetrapoda; mammalia; eutheria; primates
XX
SZ  ca. 1214 bp
XX
LO  2
DE  G000205; apolipoprotein B
DP  Direction: 5'; 5262 - 4048; Pos 1: ATG
DN  Internal: n; Gene position numeral: 1
DC  positions refer to an Xba I-Nco I fragment [1];
DC  numbering system from Ludwig et al., DNA 6: 363-372 (1987)
DC  was used [1]

G000205;
apolipoprotein B

XX
SF  AAACAAAAAA motif in the antisense strand [1]; AAAATAATTT
SF  motif in the sense strand [1]; overlaps with repetitive
SF  sequence [1]
XX
CH  border of the DNase I-sensitive domain [1]
XX
FF  confers insulator activity to single copy transgenes [2];
FF  caution: in a reinvestigation either this or the more
FF  proximal S/MAR failed to bind in standard assays [3]
XX
CP  HepG2, CaCo-2 & HeLa [1]
XX
EV  in vitro selection of S/MAR [1]
EC  matrices from HeLa, HepG2 & CaCo-2 cell lines
EV  in vivo selection of S/MAR [1]
XX
RN  [1]
RX  MEDLINE; 90078221 PubMed; 2592370
RA  Levy-Wilson, B., Fortier, C.
RT  The limits of the DNase I-sensitive domain of the human
RT  apolipoprotein B gene coincide with the locations of
RT  chromosomal anchorage loops and define the 5' and 3'
RT  boundaries of the gene
RL  J. Biol. Chem. 264:21196-21204 (1989)
RN  [2]
RX  MEDLINE; 95097992 PubMed; 7799927
RA  Kalos, M., Fournier, R. E.
RT  Position-independent transgene expression mediated by
RT  boundary elements from the apolipoprotein B chromatin domain
RL  Mol. Cell. Biol. 15:198-207 (1995)
RN  [3]
RX  MEDLINE; 98187656 PubMed; 9528807
RA  Namciu, S. J., Blochinger, K. B., Fournier, R. E. K.
RT  Human matrix attachment regions insulate transgene
RT  expression from chromosomal position effects in Drosophila
RT  melanogsaster
RL  Mol. Cell. Biol. 18:2382-2391 (1998)
//