AC  SM0000121
DT  5.2.1999 14:09:16 (created); ili
DT  30.1.2002 14:11:00 (updated); ili
OS  tobacco, Nicotiana tabacum
OC  eukaryota; viridiplantae; embryobionta; magnoliophyta;
OC  magnoliopsida; asteridae; solanes; solanaceae
DE  G001410; root-specific RB7-5A
DP  Direction: 3'; Pos 1: ATG
DN  Internal: n; Gene position numeral: -1

root-specific RB7-5A

SF  caution: may be one large (ca. 5.3 kb) S/MAR or several
SF  smaller ones [1]; 3 sites homologous to Drosophila
SF  topoisomerase II consensus, A and T boxes, ATC tracts,
SF  AT-rich regions and motifs homologous to yeast ARS
SF  consensus [2]
FF  stronger than Y$ARS1 SM000120 [2]; augments gene
FF  expression [2] [5]; expression shows tendency to
FF  increase with copy number up to a certain amount of copies
FF  [4]; reduces the loss of expression that would
FF  otherwise occur from one generation to the next [13687,
FF  13689]; does not decrease intertransformant variability
FF  [2]; does not (totally) protect against silencing in
FF  trans [3] [5]; no preferential MAR-enhanced trangene
FF  expression upon conversion from differentiated cells to
FF  rapidly-dividing cells in culture [6]
EV  in vitro selection of S/MAR [1]
EC  matrix from NT1 cells
RN  [1]
RX  MEDLINE; 21519305 PubMed; 11607225
RA  Hall jr, G. E., Allen, G. C., Loer, D. S., Thompson, W. F.
RT  Nuclear scaffolds and scaffold-attachment regions in higher
RT  plants
RL  Proc. Natl. Acad. Sci. USA 88:9320-9324 (1991)
RN  [2]
RX  MEDLINE; 96240023 PubMed; 8672887
RA  Allen, G. C., Hall jr, G. E., Michalowski, S., Newman, W.,
RA  Spiker, S., Weissinger, A. K., Thompson, W. F.
RT  High-level transgene expression in plant cells:effects of a
RT  strong scaffold attachment region from tobacco
RL  Plant Cell 8:899-913 (1996)
RN  [3]
RX  MEDLINE; 99005255 PubMed; 9790594
RA  Vaucheret, H., Elmayan, T., Thierry, D., van der Geest, A.,
RA  Hall, T., Conner, A. J., Mlynarova, L., Nap, J. P.
RT  Flank matrix attachment regions (MARs) from chicken, bean,
RT  yeast or tobacco do not prevent homology-dependent
RT  trans-silencing in transgenic tobacco plants
RL  Mol. Gen. Genet. 259:388-392 (1998)
RN  [4]
RA  Uelker, B., Allen, G. C., Thompson, W. F., Spiker, S.,
RA  Weissinger, A. K.
RT  A tobacco matrix attachment region reduces the loss of
RT  transgene expression in the progeny of transgenic tobacco
RT  plants
RL  Plant J. 18:253-263 (1999)
RN  [5]
RA  Vain, P., Worland, B., Kohli,  A., Snape, J. W., Christou,
RA  P., Allen, G. C., Thompson, W. F.
RT  Matrix attachment regions increase transgene expression
RT  levels and stability in transgenic rice plants and their
RT  progeny
RL  Plant J. 18:233-242 (1999)
RN  [6]
RX  MEDLINE; 21564978 PubMed; 11708656
RA  Ascenzi, R., Ingram, J. L., Massel, M., Thompson, W. F.,
RA  Spiker, S., Weissinger, A. K.
RT  The role of cell differentiation state and HMG-I/Y in the
RT  expression of transgenes flanked by matrix attachment
RT  regions
RL  Transgenic Research 10:465-470 (2001)