S/MARt DB - S/MAR

AC  SM0000159
XX
DT  7.5.1999 12:16:06 (created); ili
DT  18.6.2002 13:10:00 (updated); ili
XX
NA  HPV$LCR-MAR
XX
SY  long control region MAR, 
XX
OS  HPV-16, human papilloma virus type 16
OC  viridae; ds-DNA nonenveloped viruses; papovaviridae;
OC  papillomavirus
XX
SZ  301 bp
XX
DE  G000171; human papilloma virus type 16 E6 promoter
DP  7155 - 7455; Pos 1: genome numbering
XX
SQ  GTATTGTATGTATGTTGAATTAGTGTTGTTTGTTGTGTATATGTTTGTATGTGCTTGTAT
SQ  GTGCTTGTAAATATTAAGTTGTATGTGTGTTTGTATGTATGGTATAATAAACACGTGTGT
SQ  ATGTGTTTTTAAATGCTTGTGTAACTATTGTGTCATGCAACATAAATAAACTTATTGTTT
SQ  CAACACCTACTAATTGTGTTGTGGTTATTCATTGTATATAAACTATATTTGCTACATCCT
SQ  GTTTTTGTTTTATATATACTATATTTTGTAGCGCCAGGCCCATTTTGTAGCTTCAACCGA
SQ  A
SC  EMBL #K02718; nt 7155-7455
XX
FT  53 - 83: CDP binding [2]
FT  69 - 99: CDP binding [2]
FT  84 - 114: CDP binding [2]
FT  100 - 130: CDP binding [2]
FT  146 - 176: CDP binding [2]
FT  162 - 192: CDP binding [2]
FT  177 - 207: CDP binding [2]
FT  193 - 223: CDP binding [2]
FT  195 - 267: mutations reduce affinity to nuclear matrix
FT               [1]
XX
SF  contains motifs with similarity to the topoisomerase II
SF  consesus of Drosophila and chicken [1]
XX
BP  strong [1]
XX
FF  acts in transient transfection as cis-responsive repressor
FF  on the E6 P97 enhancer-promoter [2]; weak
FF  cis-responsive activator in stable transfectants [2]
XX
CP  SiHa, HPK I, HPK II cells [1]
XX
EV  in vitro selection of S/MAR [1]
EV  in vivo selection/LIS [1]
XX
BF  SB000079; CDP [2]
MM  direct gel shift; 
PR  several 31-mers representing the whole MAR [2]
SO  HeLa; human
QA  6
BF  SB000079; CDP [2]
MM  gel shift competition; 
PR  probe represents pos. 7209 to 7239 of the virus genome
PR  [2]
SO  HeLa; human
QA  6
XX
DR  EMBL: K02718; PA16
XX
RN  [1]
RX  MEDLINE; 98216720 PubMed; 9557642
RA  Tan, S.-H., Bartsch, D., Schwarz, E., Bernard, H.-U.
RT  Nuclear matrix attachment regions of human papillomavirus
RT  type 16 point toward conservation of these genomic elements
RT  in all genital papillomaviruses
RL  J. Virol. 72:3610-3622 (1998)
RN  [2]
RX  MEDLINE; 20148940 PubMed; 10684263
RA  Stuenkel, W., Huang, Z., Tan, S.-H., O'Connor, M. J.,
RA  Bernard, H.-U.
RT  Nuclear matrix attachment regions of human papillomavirus
RT  type 16 repress or activate the E6 promoter, depending on
RT  the physical state of the viral DNA
RL  J. Virol. 74:2489-2501 (2000)
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