S/MARt DB - S/MAR

AC  SM0000408
XX
DT  13.2.2002 11:22:00 (created); ili
DT  3.9.2002 16:56:00 (updated); ili
XX
NA  HS$TCRdeltaE-5MAR
XX
OS  human, Homo sapiens
OC  eukaryota; animalia; metazoa; chordata; vertebrata;
OC  tetrapoda; mammalia; eutheria; primates
XX
SZ  1022 bp
XX
DE  G000395; T-cell receptor delta
DP  Direction: 5'; Pos 1: delta enhancer
XX
SQ  TCTAGAAGAACTACACTAAGAGTTGGAGCATTCAGGTGCAAGGGAGACAGTGGGAGGGAT
SQ  ACTTCAGCCCCAATTCCTCAGGAAGCTGCTTCATCTCTGCACATCCCTTTGGCTAAGCTA
SQ  ACTAGTATGCGTGCCTCCTTGCAAACTGAAAACAAGTGTCCTACAGCTTGGAAGATTCTG
SQ  GAGAAAGGTAGTTAGCATGGTCAGAGTAATTAGGTAGAACAAACTTTTATTTCAGGGTTT
SQ  AAGTTAAATCCCATATTGGCAGAAAGTCTTGAAATTCTGAGTGACATTTATTACAGTTGT
SQ  TGCAATTCTTAGGGGATCAGTAGGACTAGCTATGAGAATCTAAAACATAGGTGACTTCTA
SQ  AGTTTATAAATTAAGAAAGACTGTTCAGGAATGGTACACACTTTTTTGGTATTGTTTACC
SQ  AGCACATAAACTTAGCAATGTATTGGTGTTCATAAAAAGAGAAAGTCTGACAATAAAGCA
SQ  ATTTTCCAAAGAAATAACACTTGAAACACATGTTGCAATGTAATTATATTTGTGGACTAA
SQ  GAAAAAAAGACTACAAGTTCTTCAAAGATAAACGATTAAATCTTTAATCTAAATTCTGGA
SQ  CTACAATTTCATACAGCTTCACGTCTGGGTTTAAGTCAAATACAAAATAGACTAGGATGC
SQ  ATATGGCAACTATATTGAGTGCACTCCAGTTTTTAAAAAGGTTTAATTCAGTCAGACACC
SQ  CATTTCTTTGGTTACCAAGATGCCAAACAATGGATTCTATAAGACACTTTCTTTCAAACA
SQ  ATCGTTTTGAAAATATAAACATTAATGTAGAAAGAGGGATTCAGAAAAATGTTTTATCAA
SQ  ATCAATGTATTATGCTCTATCTGAAATGCTGACTAACAGAGTCAGAGCACAATATCATAA
SQ  GTGCATGACCTTCTTCAGACATCTGTCTTTTCAGTTATATTGTATTATTCAAATTACTCA
SQ  AACCCAATCTCCCCAGCCCAAAGTGATGATTTGACAACTGCCTGATGAATTTAGCTTTTA
SQ  AA
SC  EMBL #AE000661; nt 213387-214408
XX
FT  795 - 809: Drosophila topoisomerase II consensus site
FT               (14/15) [1]
FT  798 - 812: Drosophila topoisomerase II consensus site
FT               (13/15) [1]
FT  838 - 852: Drosophila topoisomerase II consensus site
FT               (13/15) [1]
XX
SF  stretches with A/T/C sequence context [1]
XX
FF  adjacent to enhancer [1]; stronger than SM0000409
FF  [1]; suggested to have major influence on the delta
FF  enhancer-dependent V(D)J recombination in single copy
FF  transgenes, but to have little or no influence on these
FF  event in multicopy integrants [1]
XX
EV  in vitro selection of S/MAR [1]
EV  in vivo selection/LIS [1]
EC  tested fragment contains both MARs surrounding the TCR
EC  delta enhancer and the enhancer itself [1]
EP  3.8 kb Bam HI-Bgl II fragment
XX
DR  EMBL: M94081; HSTCRADCV
DR  EMBL: AE000661; AE000661
XX
RN  [1]
RX  MEDLINE; 99449792 PubMed; 10518560
RA  Zhong, X.-P., Carabana, J., Krangel, M. S.
RT  Flanking nuclear matrix attachment regions synergize with
RT  the T cell receptor delta enhancer to promote V(D)J
RT  recombination
RL  Proc. Natl. Acad. Sci. USA 96:11970-11975 (1999)
//