S/MARt DB - S/MAR

AC  SM0000435
XX
DT  25.6.2002 15:01:00 (created); ili
DT  13.2.2004 16:50:00 (updated); ili2
XX
NA  ARI$RRNA-MAR
XX
OS  silkworm, Attacus ricini
OC  Eukaryota; Metazoa; Arthropoda; Tracheata; Hexapoda;
OC  Insecta; Pterygota; Neoptera; Endopterygota; Lepidoptera;
OC  Glossata; Ditrysia; Bombycoidea; Saturniidae; Saturniinae;
OC  Attacini; Attacus
XX
SZ  1405 bp
XX
DE  G004507; gene encoding the 18S rRNA
DP  Direction: 5'; Pos 1: transcriptional start
DN  Internal: n; Gene position numeral: 1
DE  G004508; gene encoding the 28S rRNA
DN  Internal: n; Gene position numeral: -1

G004508;
gene encoding the 28S rRNA
G004507;
gene encoding the 18S rRNA

XX
SQ  GGGTGTTGTGCTCGGTAGAGCAGCGTCGTGCTGCGATCTGTTGAGACTCAGCCCTACGCC
SQ  AGGTGATTCGTCCGAGGACGATGACATACTTTGTTGACGATTTTGTTCGTTTTCATTATA
SQ  TTATATTTTATATATATTCATTACGCGAACGGTCGCAACGGCTTGTCTTCATCTCGTAAC
SQ  GCGTGTGTCAGATTTACCATTACGTGAACTCCCCGCGAGATTACATTTTTTATTTAACTC
SQ  TCTCTGTACGGTTTTGTGATATATATATTTATTTCATTTCATTTTCATCTCATACCGTAC
SQ  GATCGTTACGTTACAACGGATAAATGGTAATAAAACATATTCGAAATCTGCGACACGTGG
SQ  ACGCTCGCCGGCCGCTACCGCGGCCGTCTCGCTTTGTAAGTTTCCCGCTAAAGCAAACCT
SQ  AACCTAACCAACTCCGGCTAGAGGTCGAGCAGCGAGAACGTCTCCTCCGCCGAGAACGTA
SQ  ATATTGAGCGAGAACGTCATCTCGGTCGTCTTTAATTTGTTAAACGGATAAACGTATAAA
SQ  AACCTATAAAAATAATATATCTATATATATATATATAGCGAGGGATACGTTCCGAAAAAA
SQ  TCGCGTTAAAACTGAAAATCGCTAGTATTATGAGAGGTTTACATAGGATTATTAAACCAT
SQ  AGGTACAAAATTTAATACCAAAAAAAATACCAAATCATTTTTAATCATACACTCTTTAAT
SQ  TCTCTAAGAGAACCACTTTTATGGGAATACCATACCTTCTTATTTTTTAACGGAAAAATA
SQ  CATCTATTGTCTTTACATTATGTTTATATGCACTCTTTCGCGTAATGCGAGGCATTTGAG
SQ  CGCGAAATTTCAAATTCGCATTATAGTGAAACCGCGCGTAACGGGTTCGCGCTAAGCGGG
SQ  CTATTTTTTTATATATATATATATATATATATATATATATATATATATTATACTATAATA
SQ  ATAAATATGTTAGTAATATAAACGGACCTCGATTTTAATAAAGCAAATATAACAACAATA
SQ  AAACTCTCCGTTTTATCATACCACCGACACAGAAATAATCCTTTGTAGACTGTCCATAAT
SQ  TAATTGAATAGCTGTTCAGACTATGGACCGGTTATTTTTTTTTTTTTTTTTTTTTTTTTT
SQ  TTTTTTTTTTTTTTTGTTATAATCGTATATTAATCATGTAATAATAATTACCTAAATGTA
SQ  AATTTATAAAAATAAATTGTCCGACACACTTGCGACACAAACCACGGGCGTAATCGAAAA
SQ  ATGAATGATCTCCCGCGATACAGGAATCAACATTCCCAGTGCGGGGATCAATAGACACGT
SQ  ATGTGTGTAGACACATATTTGTGCGTTAAATTTGTAAAAATAATAAAGTTTTCTGTATTA
SQ  TGTATTTGTTTTGTGAATAGAATTC
SC  EMBL #AF463459; nt 9103-10324, edited according to [4]
XX
FT  1064 - 1331: major enhancement of transformation frequency
FT                 in S. cerevisiae [4]
FT  1064 - 1331: ARS activity in Saccharomyces cerevisiae
FT                 [4] [2]
XX
SF  contains DNA unwinding regions [4]; contains multiple
SF  ARS consensus sequence motifs [4]; contains inverted
SF  repeats and palindromic sequences [1]
XX
FF  enhances gene expression in stable transformed cells but
FF  not in transient transformed cells [3]; enhances
FF  transformation frequency in Saccharomyces cerevisiae [4]
XX
EV  southwestern blotting [3]
EC  LIS treated nuclear matices of murine 3T3 cells were used
EC  [3]
EV  in vitro selection of S/MAR [2]
EC  binding to S. cerevisiae nuclear scaffolds in the presence
EC  of lambda DNA [2]
EP  Acc I-Acc I-fragment [2]
XX
DR  EMBL: AF463459; AF463459
XX
RN  [1]
RX  MEDLINE;  PubMed; 12232588
RA  He, M.-L., Zhao, M.-J., Jin, J.-R., Li, Z.-P.
RT  The DNA sequence and structural characteristic of the
RT  5'-nontranscribed spacer of silkworm Attacus ricini rDNA
RL  Acta Biochim. Biophys. Sin. 28:616-623 (1996)
RN  [2]
RX  MEDLINE;  PubMed; 12174275
RA  He, M.-L., Zhao, M.-J., Li, Z.-P.
RT  The autonomous replication function (ARS) of the
RT  scaffold-associated region (SAR) of silkworm Attacus ricini
RT  rDNA in yeast
RL  Acta Biochim. Biophys. Sin. 30:147-153 (1998)
RN  [3]
RX  MEDLINE;  PubMed; 12053190
RA  Wu. Z.-Y., Zhao, M.-J., Li Z.-P.
RT  Regulation of Gene Expression by SAR of Silkworm Attacus
RT  ricini rRNA Gene
RL  Acta Biochim. Biophys. Sin. 33:59-64 (2001)
RN  [4]
RX  MEDLINE; 22358399 PubMed; 12470638
RA  Chen, Y., Zhao, M., Li, Z.-P., He, M.-L.
RT  The function of the nuclear matrix attachment region of
RT  silkworm rDNA as an autonomously replicating sequence in
RT  plasmid and chromosomal replication origin in yeast
RL  Biochem. Biophys. Res. Commun. 299:723-729 (2002)
//