S/MARt DB - S/MAR

AC  SM0000490
XX
DT  17.2.2004 18:05:00 (created); ili2
DT  10.3.2004 16:08:00 (updated); ili2
XX
NA  HS$CD8B1-3MAR6
XX
OS  human, Homo sapiens
OC  eukaryota; animalia; metazoa; chordata; vertebrata;
OC  tetrapoda; mammalia; eutheria; primates
XX
SZ  5814 bp
XX
LO  2p11
DE  G001435; CD8 antigen, alpha polypeptide
DP  Direction: 5'; Pos 1: transcriptional start
DN  Internal: n; Gene position numeral: 1
DE  G001436; CD8 antigen, beta polypeptide 1
DP  Direction: 3'; Pos 1: transcriptional start
DN  Internal: n; Gene position numeral: -1

G001436;
CD8 antigen, beta polypeptide 1
G001435;
CD8 antigen, alpha polypeptide

XX
SQ  CCCGGGTTCAAGCAATTCTTCCACCTCAGCCTCCCCAGTAGCTGGGATTACAGGCGCCTG
SQ  GCATCATGCCTGGCTAAATTTTGTATTTTTGTAGAGACAGGGTTTCACCATGTTGGCCAG
SQ  TCTGGTCTTAAATAARTTGTGCGTGTGTGTGTGTGTGTGTGTGTGTTTGTGTGTGTGTGT
SQ  GTTTAAAGCTTACAAATACATGAATATAAGTTTTGTCAGAAAAAATATAATTAATATTTC
SQ  TTTGCTTTCCCAAATCTGCCATCAGTTTGTAAAAACACACAGTTTGTATAAACCTAGAGA
SQ  TTTTTCTCTGCATTTAGACACACATTTTTCTGTGCATTTAGACATTCACACACAGACACA
SQ  CACACACACACACGATTTTTACCTAAAAGAGTTATAATATATATATTGGTCTGCAACTAG
SQ  CTTTTTTTACTTGACAATATATGTCAGAATTTGCTATATTCCAGGACATATAGATGTATT
SQ  GGCTTATGTTTGACTGCCTCCTGGTATTCCTGTAGGTCGGTGAACCATAATTTAAATTCC
SQ  TATTGCCCCATTGATGAAGACTGGGTTTTCCCACTTTAAAGGCAGTGCTGCATGAACACC
SQ  TTCGCACCTGCCTTATTATGCACAGGTATGAGGAAGTATATTTTGCTGCACACAGAGATA
SQ  GACATGATACTGGTTGAGAGCTCAGGCAATCTTTGGGAAAACGTTGATAAACAGTCATTA
SQ  CAATGGACGGGACAGGGGAAAGAAGAGACAAAAAACATCTGTAACAAATAGAAAACAGTT
SQ  ACGAACATAGTAGATACTAATCCAACTATATCAATAACCACTTTCAATGTGAATGGTCTA
SQ  AATATATCCAAGTAAAAGACAGAGATTGTCAGAGTAGTAAAAAGCNAGGCNTTGTAAAAC
SQ  GACTCCACTANTAGGGGGATTGGCCCAAGTCGGCCGNGCTNGAATTNGGCGNGGGCGGGC
SQ  CGCTNCNGATGTTTTAANTAAGNTGGNACCTAGTCGANAAGGCCTAACCATATGCTGTCC
SQ  ACAAGAAACCCACCTTAAATGTAAGACTCAGATGGGTTATAAGGAATAGAGAAAGATATA
SQ  TCTTGTTAACACTATCAAAAGAAAGCTGGAGTAGCTAATTTCAGACAAAGCAGACTTCAT
SQ  AGCAAGGAAAATCATCAGGGATAAAGAAGGACATTACATAATATTAAAAGGGTCAATTCT
SQ  CCAAGAAGACAAAACAATCCTTAACATGCATACATGTATTAGCAAAGCATCAAAATACCT
SQ  GAGAGAAAAAATGATAGGTATGCAAGGAAAAATACACAAAGTCACTATTATAGTTGGATA
SQ  TTTCAATACTTCTCTCTCAATAATTGATAAATCTAGCAGGTAGAAAGTCAGTAATGATTT
SQ  AATTGACCTCAACAGCACTCTCAACCAGTTTGATNTAATTGACATTTATAGAATACTCTA
SQ  TCCAACAACAAAATACATATTCTTCCAAGTTCACATGGAACATTTACCAAAAGAGATTGC
SQ  ATTCTTTCCCATAAAACACACTTTAACAAACTGAAATGAACANGAAATCATATAAAGTAT
SQ  GCTGTCMGACCACAACAAGAATTAAACTAGAAATTWATTAACAAAAAGAGAGCTGAAAAG
SQ  TCTCCAAATATTTGAAGATTAAACAACATGCTTCTAAATAACACATGGGTCAAAGAAGAC
SQ  GTCTCAAGAGATATTTTAAAATATTTTTTTCAGTGTTAAGANTTTATTGTNCGCCCACAA
SQ  GCAATGCAGTACTTNGAGGGAAACTTATAGCATTAAATACACATATTTTGAAAAGAGATC
SQ  TAAAATTGACCATCTAAAATTCCACCTTAGGAAACAAGAGAAGGAAGAGCAATACAAGCC
SQ  TAAAGCAAGCAGAAGAATAAAAATAATAAAAATTAGAGAAATCAATGAAACTGAAAACAG
SQ  GAAACAATAGAGAAAATAAAACAAAAGCTATTTCTTTGAAAAAGATCAGTAAAATTGGTA
SQ  AACCTCTAGTCAATCTAACCAAGAAAAAAGAGAGAAGGCAAAAATTACTCATATCAGAAA
SQ  CAAAAGAGAGATCATCACTATGGATCTCACAGACAATAAAAGGATACCGAAGGAATATTA
SQ  CAAATGAATGGGGCCGCTTGTGTATGGCAAAGCTCGGCTATTCCTTTTCTTTCTTATCCT
SQ  GAAAGAAGGCAAAGGGTCCTAACTCTCCATACTACTTTCCCTTCCTCACCACCCCACTCC
SQ  CTTTCTNTGATGGAAATTGAGACATGAATCTATCAATCCCAGGAAAATCATGTAATCTCA
SQ  CTGGGCATTATTTTTCCCCATATTTTAGCCAAAATATGCTAAAATCTCACTACTCAAAAA
SQ  AAAATGGTTAAAAGAAGTTTCCCAAATTTGCATAAGCTTTAGGCCCCATAGGAACTCTAT
SQ  CTGCTTTGTTTGCCACACTGAAATTTCTGCTAAGATCCAGGGGGAGCAGGGTCAGTCAGG
SQ  GTGAATTTGAGGCATCTGTTTGGTGTGCTGGAGTTTTTCTTTTTCAATAAGGTGTTTTGT
SQ  GGTAGAAGAAATAGCAATGTAATTCCTACAGTGTTAAGAGCTGAAAGAGGGGGTTTNAGA
SQ  AGGAAGTTCAAACTNCTNCTNANAAAGACATTAACCTCCTTCAGTTAAAAGTTAGAAAAC
SQ  AGAAGCACAAAAGGACAAGAACAGACAATCCACACACAAAAAAGAAAATTCAAGTGATTA
SQ  CAGATCTACAGAAAAACCGTCAACCGCAATAAAAATCAAAGGAGATTGTAAAAATTATCC
SQ  CACTTCTTGCTTCTCCCAAAATAACACTGATGCATGTACACACACACACACACACACACA
SQ  CGCACACACACCACACATACTAAGTCCTCACTTAACATCATGTATAGGTTCTTGGAAACT
SQ  GTGATTTTAAGCAAAATAACATACAGCAGATCCTCAAAAAGTATTTTTTCATTCAATGTT
SQ  GTTTTGTTACAATGTTGATTGAAAAACTGGTTTTATTATATGTTGTTTGGCTTAAAGTTG
SQ  CAGTTTCCAAGAACCTATGGATGACAGTGAAGACTTACTATATGCGTGTATCTGTGTGTA
SQ  TGTGTGTGTGTGTGTGTAATTTGTTTTTTAATTCCTGGGGCTGACCAGGGGTTAGTGAAA
SQ  GAAGAACTCTGATGCAGCCAACAGTATACATCTGTCAAATCTTTAAAAGGCCAACTTGCC
SQ  TTTAAGAGCCATTTCCTTCTAAAAATTCAAACCAAGAAAATAATTCAAATGCACAAAAAG
SQ  ATATATAAGCCAAAATGTTTCTTTGTTTATTACTTTAACAGCCATATGGTTTTTATACTC
SQ  CATAACATCACTTTAAAATATATAATGCACAAACTGTTAGAAATATAAACAAGAGGCCAG
SQ  GCGCGGTGGCTCACCTCACGCCTGTAATCCCAGCACTTGGGAGGCCGGGGTGGGCGGATC
SQ  AAGAGGTCAGGAGATGCAGACCATCCTGGCTAACACGGTGAAACCCCGTCTTTACTAAAA
SQ  AATACAAAAAATTAGCCGGGTGTGGTGGTGGGCGCCAGTAACCACAGCTACTCGGGAGGC
SQ  TGAGGCAGGAGAATGGCGTGAACCCGGGAGGCGGAGCTTGCAGTGAGCCGAGACCACGCC
SQ  ACTGCACTCCAGCCTGGGCGACAGAGCGAGACTCCGTCTCATAAAAAAAAAAAAAAAATA
SQ  AAAAATTNTATATATATATATATAAAGAAGAAATAGTAAATCCACAAACACATTTAAAAA
SQ  ACTTACAGNTCTTTCAAAAATTAGATCAAGTAGATAAAAATTTTAAAAAGACATAGCAGA
SQ  TTTGGATAATACATTTTACAAAGCTTGATCTAATWTAAAGAATTTTGTGCCCAATTCTTT
SQ  TATACAAAACTGGGCCTAAACTTATTTTCGAAATCAACCTTCAATCAAAAACTTCAATTT
SQ  CAAAAAGCGGTAAATACAGCCACATTTTCAGACCACAGAGCAATAAAATTATAATCCAAA
SQ  GAAGGGGGAAAAAATGTATCCACTTAAAAATTTGTTATCACCTTTTAAGAACTGTTATGA
SQ  TAACTAAGAAACAAAAATTGAAATTATACACTATTTAGAAATTGCTGTATCATAATTTAA
SQ  AAAGTACACACGACCTTAATGTCTAATTGTGCAAAGTATGAGTGGAATATTATGCAGATA
SQ  TCAGATAAGATTCATGAAGAACGTGTTATAAAAGAAATTGGCTGAAATAAAATTTTTATG
SQ  GAACTTTATGATTAAAAAATTTTTAAAGCAGGYTRGAGACYTKAWTRKCCGMMCAYAATG
SQ  ATGWSWGKGMTGGAMAAAATAGTTRRAAATATATAAGCATAGAATAGAAACAAAGCAAAT
SQ  GCAAAACAATATACCAAAATGTTAGGAGTGACTATAATTCCATATTGGCACTATAAGCCA
SQ  TTATTTTTGTTTTGTTCTGTTTTCTATTCTCCAAAATATTTTTAACCTAAAAAAGACAGC
SQ  AAAAGTGAGGCAGAGAGGAATTTAGGGACTTGCTAAAGTCMCAAMTWYWWTGYMCGGCCC
SQ  ASRCCACCTSYSRGCKKMCAWTRMWGTSTCTAAWCAGCCCGGGACTTCAGTAAACGACAA
SQ  CAACAACAACAAAAAACGAGGATTCACATCACCAGGAGAGAAACGGGCTCGGCCTAAGGA
SQ  CTTGGTAGTAGCTGGCAGCAGGACGCGCTTCGACTAAGGGGCTCTGGTTCCAAGCACCCC
SQ  CAGAGCCAGCGCAGGAGAGGACCTTCCAGGTAAACTAAAACCAAGAGCGGAATGAAATCG
SQ  GTGAATAAGCCAAACGAACCTCAGAGGGACCTTGGCACCCGCACCCCCGCCCCGCACCTT
SQ  AGTTCTGCGAGCCTTTCCCCGTCTCCAACGCGGGCAAGGCTGTCAGATCCTCAAGCCGAC
SQ  CCTTCTCTGCACCGCGACCTCAGTGTCCTGCGCGGGACATCAGAGGCCGGACGCTCCACC
SQ  TGCCCCAGGGCACGCCCCGAGCCCCCAAAGCCCATCTCCTTCCTGTCGGGTGCTCACCTC
SQ  TTCCTCCCTGCGGGCCCAGGGCCTTCTTGAGGGGCTGCGGATGCGAATGAGCCAGCGAAG
SQ  GGCCGCCCGCGGCCCCTCGGCACCTCTGCCCAGCCGTCCCGACCAGGGGACCAGCTCAGC
SQ  CCCGGTCGGGGCCCCGGCACCCAGGACCGCTCAGGATCTCCATTCCAGYCCCTTCGCACT
SQ  TTCTGACGGGGAAAGGAGAGTTGGGARAACSAGACTCTCAAGTTYTGGGGACTTCTACCC
SQ  ACCATTAAAGCTTTTGGCATTCTGGCCATTTCTTTCTTTTTAAAAAAATWATWATTAWTA
SQ  TTTTTTATTTTGANGAWGGGAGTCTYGCTTYTATCCCCCARCCTGGGAGTGCAGTGACTC
SQ  GATCTCCGCTCGCTGCAACTTCCGCCTCCAGGGTTCAAACGATTCTTCTGCCTCAGCCTC
SQ  CCCAGTAGCTAGGACTACAGGCTTGCGCCATCACGCCCGGCTAGTTTTTTTTTTTGTTTT
SQ  TTGTTTGTTTGTTTGTTTGTTTTTAAAGTAGAAACAGGATTTCGCCATATTGGCCAGGCT
SQ  GGTCTCGAACTCCTGACCTCAGTTGATCCGACCGCCTCGGCCTCCCAAAGTGTTGGAATT
SQ  ACAGCAGTGAGCCACCGCGCACCAAGCCCGGCCCATTCTGGCCATATCTATAGACGGATG
SQ  CATTGCACAGGAGGCTCAGCACTAATCGGTAGATACTGCGAGATGCTGGGAGGTTAAGGG
SQ  GCCTACCCGCAATATCTCTGGCMAATGCCTTKGGSTAGAAAATKCCATAANTTAGCCGNY
SQ  TCTTTTRATCCCTTGCAAAATGCGAATTCCACCGSACCTYMACCCCANCSSGRG
SC  EMBL #AY03272; nt 9065-14878
XX
FT  1315 - 1536: SATB1 binding [1]
FT  3735 - 3759: GATA3 binding [1]
FT  4128 - 4159: GATA3 binding [1]
XX
SF  comprise T cell-specific DNase hypersinsitive sites [1];
SF  contains LINE/L1, Alu, MER69 and MER6B elements [1]
XX
FF  considered as 2 strong MARs flanking a weaker one in [1]
XX
EV  re-binding assay (high salt) [1]
XX
BF  SB000012; SATB1 [1]
MM  direct gel shift; 
SO  rec(human-); human
QA  6
BF  SB000012; SATB1 [1]
MM  assessment of the dissociation constant; 1.6-2 nM
PR  subfragment
SO  rec(human-); human
QA  6
BF  SB000096; GATA-3 [1]
MM  direct gel shift; 
SO  Jurkat; human
QA  6
BF  SB000096; GATA-3 [1]
MM  supershift (antibody binding); 
SO  Jurkat; human
QA  6
BF  SB000096; GATA-3 [1]
MM  gel shift competition; 
SO  Jurkat; human
QA  6
XX
DR  EMBL: AY032722; AY032722
XX
RN  [1]
RX  MEDLINE; 21935500 PubMed; 11937547
RA  Kieffer, L. J., Greally, J. M., Landres, I., Nag, S.,
RA  Nakajima, Y., Kohwi-Shigematsu, T., Kavathas, P. B.
RT  Identification of a candidate regulatory region in the
RT  human CD8 gene complex by colocalization of DNase I
RT  hypersensitive sites and matrix attachment regions which
RT  bind SATB1 and GATA-3
RL  J. Immunol. 168:3915-3922 (2002)
//