S/MARt DB - S/MAR

AC  SM0000501
XX
DT  23.2.2004 15:25:00 (created); ili2
DT  23.2.2004 16:53:00 (updated); ili2
XX
NA  Y$HML-5MAR
XX
OS  yeast, Saccharomyces cerevisiae
OC  Eukaryota; Fungi; Ascomycota; Hemiascomycetes;
OC  Saccharomycetales; Saccharomycetaceae; Saccharomyces.
XX
SZ  601 bp
XX
LO  3
DE  G009226; 
DP  Direction: 5'; Pos 1: transcriptional start
DN  Internal: n; Gene position numeral: -1
DE  G009227; 
DP  Direction: 5'; Pos 1: transcriptional start
DN  Internal: n; Gene position numeral: 1
DC  locates to the intergenic region between the alpha 1 and 2
DC  genes [1]

G009226;
G009227;

XX
SQ  TTTAAACTCATCTGTGATTTGTGGATTTAAAAGGTCTTTAATGGGTATTTTATTCATTTT
SQ  TTCTTGCTTATCTTCCTTTTTTTCTTGCCCACTTCTAAGCTGATTTCAATCTCTCCTTTA
SQ  TATATATTTTTAAGTTCCAACATTTTATGTTTCAAAACATTAATGATGTCTGGGTTTTGT
SQ  TTGGGATGCAATTTATTGCTTCCCAATGTAGAAAAGTACATCATATGAAACAACTTAAAC
SQ  TCTTAACTACTTCTTTTAACCTTCACTTTTTATGAAATGTATCAACCATATATAATAACT
SQ  TAATAGACGACATTCACAATATGTTTACTTCGAAGCCTGCTTTCAAAATTAAGAACAAAG
SQ  CATCCAAATCATACAGAAACACAGCGGTTTCAAAAAAGCTGAAAGAAAAACGTCTAGCTG
SQ  AGCATGTGAGGCCAAGCTGCTTCAATATTATTCGACCACTCAAGAAAGATATCCAGATTC
SQ  CTGTTCCTTCCTCTCGATTTTTAAATAAAATCCAAATTCACAGGATAGCGTCTGGAAGTC
SQ  AAAATACTCAGTTTCGACAGTTCAATAAGACATCTATAAAATCTTCAAAGAAATATTTAA
SQ  A
SC  EMBL #X59720; nt 12962-13562
XX
SF  note: shown is the approximate sequence containing the MAR
SF  as infered from [1]
XX
FF  overlaps with the P regulatory domain of the HML locus
FF  [1]
XX
EV  re-binding assay (LIS) [1]
XX
BF  SB000097; RAP-1 [1]
MM  direct gel shift; 
SO  yeast, extract; baker's yeast, Saccharomyces cerevisiae
QA  6
BF  SB000097; RAP-1 [1]
MM  gel shift competition; 
SO  yeast, extract; baker's yeast, Saccharomyces cerevisiae
QA  6
XX
DR  EMBL: X59720; SCCHRIII
XX
RN  [1]
RX  MEDLINE; 89249338 PubMed; 2655930
RA  Hofmann, J. F., Laroche, T., Brand, A. H., Gasser, S. M.
RT  RAP-1 factor is necessary for DNA loop formation in vitro
RT  at the silent mating type locus HML
RL  Cell 57:725-737 (1989)
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